Veterinary Microbiology Questionbank -2

COMPILED BY:
SHEIKH FIRDOUS AHMAD
Alumni, Faculty of Veterinary Sciences & Animal Husbandry
(F.V.Sc. & A.H.), SKUAST-K,
Srinagar Kashmir.
E-mail: firdous_003@yahoo.com
firdousa61@gmail.com
Cell: 09469691352

  1. In Streptococci chain formation is due to the cocci dividing in _____________Plane.
  2. Toxin is employed in diagnosis of Scarlet fever____________.
  3. Selective media for Streptococci is________.
  4. Woman’s curling hair type of growth is characteristic of __________.
  5. The extracellular toxins of B. anthracis are composed of _______, ________ and _________.
  6. In anthrax-affected animals the spleen revealed ________ consistency.
  7. _______ Phage is commonly used to identify anthrax bacillus.
  8. Lancefield classification is mainly based on          antigen of the cell wall.
  9. The CAMP test is based on the partial haemolysis of the ______ toxin of Staphylococci.
  10. On Blood agar Staphylococcus releases V factor which favours the growth of ______ organism.
  11. All Clostridia are motile except _________ .
  12. All Clostridia are non capsulated except ________.
  13. Selective media for Clostridia is ___________.
  14. Based on the growth in Robertson cooked meat media Clostridia classified into______ and _______.
  15. Western duck sickness is caused by _______________.
  16. Cl. botulinum toxin produces characteristic__________ appearance in mice.
  17. Strangles is caused by ________________.
  18. Botryomycosis in horse is caused by _________.
  19. Staphylococcus hyicus causes ____________ in pigs.
  20. Selective media for staphylococci is _____________.
  21. Clostridium species ________ and ________ produces stormy fermentation in litmus milk.
  22. Malignant odema in cattle is caused by ____________.
  23. Cl. chauvoei type B causes _________ in cattle.
  24. Struck in sheep is caused by __________.
  25. Cl. welchi ferments lactose and produce acid clot in litmus milk is called as_________.
  26. Braxy in sheep is caused by _____________.
  27. Bacillus anthracis capsule is rich in _______.
  28. Sudan Black B stain is commonly used to demonstrate _________ of B. anthracsis.
  29. In gelatin stab growth of B. anthracis resembles ________appearance.
  30. Clostridia produce acid and clot in _______medium.
  31. In gelatin stab culture, Cl. tetani produces __________ type of growth.
  32. Listeria exhibits characteristic _______ motility in 24hr broth culture, incubated at 25°C.
  33. The haemolysin of L. monocytogenes is known as _____________.
  34. Neural form of listeriosis is called as ___________________.
  35. Listeriosis abortion typically occurs in ____________ trimester of pregnancy.
  36. Isolation of listeria requires a process called ________________.
  37. Swine erysipelas is caused by __________ .
  38. Luxurient growth of M. tuberculosis on LJ medium is known as ______.
  39. The cell wall of the Mycobacteria composed of ______, ______ and ______ .
  40. Haematogenous spread of tubercle results _______ form of tuberculosis.
  41. Urticarial form of erysipelas infection in pig is called as___________________.
  42. In gelatin stab culture E. rhusiopathiae gives _________ type of growth.
  43. Caseous lymphadenitis in sheep is caused by __________.
  44. To demonstrate Corynebacterium granules ________ and ________ stains are commonly used.
  45. All Corynebacteria are Urease positive except ______________.
  46. Two pathogenic species of Nocardia are ________ and __________.
  47. Bovine farcy is cause by __________________.
  48. The cell walls of Mycobacteria are rich in ______ .
  49. ________ is called as Timothy grass bacillus.
  50. The acid-fast property of mycobacterium was discovered by ______________.

Write True or False

  1.  Streptococci are catalase and oxidase negative.
  2.  The capsule of Streptococci pyogenes is made up of polysaccharide.
  3.  When Streptococci treated with penicillin, spheroplast form will occur.
  4.  Beta haemolytic Streptococci produce complete haemolysis.
  5.  Griffith typing of Streptococci is mainly based on agglutination test.
  6. Staphylococci are not able to grow in the media containing 7-10% NaCl.
  7.  S. aureus strains from dogs produce golden yellow colonies on nutrient agar.
  8. Chemically the beta haemolysins of Staphylococci are phospholipase C.
  9.  Gangrenous mastitis in cattle is mainly due to alpha toxin of staphylococci.
  10.  Oxidase test is highly useful to differentiate Staphylococci and Streptococci.
  11.  Clostridium are anaerobic, spore forming, spindle shaped bacteria.
  12.  Clostridia are motile with monotrichous flagella.
  13.  Cl. odematiens is very strict anaerobe.
  14.  In Robertsons media proteolytic clostridium turns the meat pink colour and produce gas.
  15.  Soil is the natural habitat of Cl. tetani.
  16.  Cl. tetani is very potent invasive Clostridium.
  17.  Capsulated Cl. tetani giving the characteristic drum sticks appearance.
  18.  Cl. tetani is a non-capsulated and motile bacillus.
  19.  Stiff agar (3%) is commonly used to demonstrate swarming nature of Cl. tetani.
  20.  Streptolysin S is very potent cardiotoxic.
  21.  Staphylococci are catalase +ve and oxidase negative.
  22.  S. aureus causes bumble foot in poultry.
  23.  The word staphyle means twisted or coiled bacilli.
  24.  Tick pyaemia in 2-4weeks old lambs is caused by S. aureus.
  25.  Coagulative staphylococci are arranged in grapes like structure.
  26.  Cl. tetani is saccharolytic.
  27.  Cl. tetani spores are readily destroyed by 5% phenol or 0.1% mercuric chloride solution.
  28.  Cl. tetani serological type VI possesses peritrichous flagella.
  29.  Tetanolysin is a very potent neurotoxin.
  30.  Poultry are highly susceptible to tetanospasmin.
  31.  Ascending tetanus is more common in human and horses.
  32.  The species Cl. tetanomorphus and Cl. tetanoides produces drumstick spores as like Cl. tetani.
  33.  Administration of ATS within 4hrs of infection can prevent tetanus.
  34.  Botulinum toxins are very potent enterotoxins.
  35.  Botulism is mainly due to very rapid multiplication of bacteria at the site of infection and production of toxin.
  36.  Normal heat fixation of blood smears is sufficient to kill the B. anthracis bacilli.
  37.  B. anthracis spores are readily destroyed by 5% phenol.
  38.  In anthrax lethal toxin alone is responsible for production of local oedema and shock.
  39.  Poultry are highly resistant to anthrax.
  40.  Acute fatal form of anthrax is very common in pigs.
  41.  The Sterne vaccine contained spores of a non-capsulated a virulent mutant strain of B. anthracis.
  42.  Pulmonary anthrax is called as hide porter’s disease.
  43.  Anthracoids are motile, non-capsulated and non-spore formers.
  44.  The edges of the colonies of B. anthracis appear irregular and are known as medusa head colonies.
  45.  In bacillus, sporulation is inhibited by exposure to air.
  46.  The spores of Bacillus anthracis are wider than the vegetative form.
  47.  Bacillus species are catalase positive.
  48.  Listeria is motile at 37°C.
  49.  Listeria expresses flagellar protein only at higher temperature.
  50. L. monocytogenes does not grow in media with 10% salt concentration.

ANSWERS

1                   One or Single Plane
2                   Erythrogenic
3                   Edwards Medium
4                   Bacillus anthracis
5                   Edema Factor, Protective Antigen & Lethal Factor
6                   Black Cherry Jam
7                   Cherry Gamma Phage
8                   Carbohydrate Antigen or C-antigen
9                   Beta
10                 Haemophilus
11                 Cl. perfringens
12                 Cl. perfringens
13                 Robertson’s Cooked Meat Media
14                 Saccharolytic & Proteolytic
15                 Cl. botulinum
16                 Wasp Waist
17                 Streptococcus equi sub sps equi
18                 Staphylococcus aureus
19                 Exudative Epidermitis or Glassers disease
20                 Mannitol Salt Agar
21                 Cl. perfringens, Cl. septicum
22                 Cl. septicum
23                 Black leg
24                 Cl. perfringens type c
25                 Stormy Clot Fermentation
26                 Cl. septicum
27                 Poly-D-glutamate
28                 Spores
29                 Inverted fir tree
30                 Litmus Milk
31                 Fir tree
32                 Tumbling
33                 Listeriolysin
34                 Circling Disease
35                 2nd
36                 Cold Enrichment
37                 Erysipelothrix rhusiopathiae
38                 Eugenic
39                 Peptidoglycan, Arabinomannan, Mycolic acid
40                 Milliary
41                 Diamond Skin Disease
42                 Bottle Brush Type
43                 Corynebacterium pseudotuberculosis
44                 Albert’s neissers, ponder’s stain
45                 Corynebacterium bovis
46                 Nocardia steroids, Nocardia farcinica
47                 Nocardia farcinica
48                 Mycolic acids
49                 Mycobacterium phlei
50                 Ehrlich, 1882

Errors and Omissions may please be intimated.
Suggestions are warmly welcomed.

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